Porcine Acute Diarrhea Syndrome Coronavirus (SADS-CoV) sabon abu ne da aka gano porcine enteric pathogenic coronavirus wanda zai iya haifar da gudawa na ruwa a cikin jarirai alade kuma ya haifar da asarar tattalin arziki ga masana'antar alade.A halin yanzu, babu wata hanyar da ta dace da serological don kimanta tasirin kamuwa da cutar SADS-CoV da allurar rigakafi, don haka akwai buƙatar gaggawa don amfani da ingantaccen gwajin immunosorbent mai alaƙa da enzyme (ELISA) don gyara wannan rashi.Anan, recombinant plasmid yana bayyana furotin SADS-CoV spike (S) wanda aka haɗa tare da yanki na IgG Fc na ɗan adam an gina shi don samar da baculovirus recombinant kuma an bayyana shi a cikin sel HEK 293F.An tsarkake furotin S-Fc tare da resin sunadaran G kuma yana riƙe da sake kunnawa tare da rigakafin ɗan adam Fc da anti-SADS-CoV.Sa'an nan kuma an yi amfani da furotin S-Fc don haɓaka ELISA (S-iELISA) kai tsaye da kuma inganta yanayin halayen S-iELISA.Sakamakon haka, ta hanyar nazarin ƙimar OD450nm na 40 SADS-CoV korau sera wanda aka tabbatar ta hanyar immunofluorescence assay (IFA) da lalatawar Yamma, an ƙaddara ƙimar yanke zuwa 0.3711.Matsakaicin bambance-bambance (CV) na 6 SADS-CoV tabbataccen sera a ciki da tsakanin tafiyar S-iELISA duk sun kasance ƙasa da 10%.Gwajin-reactivity ya nuna cewa S-iELISA ba shi da giciye-reactivity tare da sauran ƙwayoyin cuta na porcine.Bugu da kari, dangane da gano samfuran maganin magani na asibiti guda 111, jimlar daidaituwar adadin IFA da S-iELISA ya kasance 97.3%.Gwajin kawar da kwayar cutar tare da nau'ikan nau'ikan OD450nm daban-daban na magani 7 sun nuna cewa ƙimar OD450nm da S-iELISA ta gano yana da alaƙa da inganci tare da gwajin kawar da cutar.A ƙarshe, an yi S-iELISA akan samfuran ƙwayar noman alade 300.Kayan ciniki na sauran ƙwayoyin cuta na porcine enterovirus sun nuna cewa ƙimar IgG na SADS-CoV, TGEV, PDCoV da PEDV sun kasance 81.7%, 54%, da 65.3%, bi da bi., 6%, bi da bi.Sakamakon ya nuna cewa S-iELISA ƙayyadaddun ƙayyadaddun ƙayyadaddun abu ne, mai hankali, da kuma sake sakewa, kuma ana iya amfani dashi don gano kamuwa da cutar SADS-CoV a cikin masana'antar alade.Wannan labarin yana da kariya ta haƙƙin mallaka.duk haƙƙin mallaka.
Lokacin aikawa: Juni-22-2021